Le SIDA au Ghana (serveur d'exploration)

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Comparison of latex agglutination, wet preparation, and culture for the detection of Trichomonas vaginalis

Identifieur interne : 000F66 ( Main/Exploration ); précédent : 000F65; suivant : 000F67

Comparison of latex agglutination, wet preparation, and culture for the detection of Trichomonas vaginalis

Auteurs : Y. Adu-Sarkodie [Royaume-Uni, Ghana] ; B K Opoku [Ghana] ; K A Danso [Ghana] ; H A Weiss [Royaume-Uni] ; D. Mabey [Royaume-Uni]

Source :

RBID : ISTEX:8AD5236D4B7150C95B188E4EA75A22BE6DA24BEB

English descriptors

Abstract

Objectives: To compare the performance of three diagnostic methods for Trichomonas vaginalis infection—latex agglutination, saline wet mount, and culture. Methods: Vaginal swabs from 3807 women attending antenatal clinics were tested for the presence of T vaginalis by latex agglutination. All positives and the following two negatives were tested by wet preparation and culture. Results: The prevalence of infection by latex agglutination was 5.4%. Using an expanded gold standard based on the wet mount and culture results, the sensitivity of the latex agglutination test was 98.8% (95% CI 95.9 to 99.9) and specificity was 92.1 (89.2 to 94.5). The kappa index for test agreement was 0.93 for latex and culture and 0.88 for latex and wet preparation. Conclusion: The latex agglutination test is a highly sensitive test for detecting T vaginalis infection. It is a simple rapid test and has the potential for use in screening and diagnostic settings.

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DOI: 10.1136/sti.2003.007864


Affiliations:


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<div type="abstract" xml:lang="en">Objectives: To compare the performance of three diagnostic methods for Trichomonas vaginalis infection—latex agglutination, saline wet mount, and culture. Methods: Vaginal swabs from 3807 women attending antenatal clinics were tested for the presence of T vaginalis by latex agglutination. All positives and the following two negatives were tested by wet preparation and culture. Results: The prevalence of infection by latex agglutination was 5.4%. Using an expanded gold standard based on the wet mount and culture results, the sensitivity of the latex agglutination test was 98.8% (95% CI 95.9 to 99.9) and specificity was 92.1 (89.2 to 94.5). The kappa index for test agreement was 0.93 for latex and culture and 0.88 for latex and wet preparation. Conclusion: The latex agglutination test is a highly sensitive test for detecting T vaginalis infection. It is a simple rapid test and has the potential for use in screening and diagnostic settings.</div>
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