Comparison of latex agglutination, wet preparation, and culture for the detection of Trichomonas vaginalis
Identifieur interne : 000F66 ( Main/Exploration ); précédent : 000F65; suivant : 000F67Comparison of latex agglutination, wet preparation, and culture for the detection of Trichomonas vaginalis
Auteurs : Y. Adu-Sarkodie [Royaume-Uni, Ghana] ; B K Opoku [Ghana] ; K A Danso [Ghana] ; H A Weiss [Royaume-Uni] ; D. Mabey [Royaume-Uni]Source :
- Sexually Transmitted Infections [ 1368-4973 ] ; 2004-06.
English descriptors
- KwdEn :
- Adverse pregnancy, Agglutination, Antenatal clinics, Birth weight, Characteristic morphology, Culture results, Culture testing, Diagnostic comparison, Diagnostic methods, Diagnostic tests, Infection, Inpouch, Inpouch culture, Kappa index, Keppel street, Kwame nkrumah university, Laboratory diagnosis, Latex, Latex agglutination, Latex agglutination test, Latex agglutination testing, London school, London wc1e, Many health settings, Medical sciences, Nucleic acid amplification, Obstet gynecol, Partner notification, Polymerase chain reaction, Predictive value, Prep, Prep culture, Prep microscopy, Prep technique, Present cost, Rapid tests, Remote communities, Reproductive health settings, Risk factors, Specimen collection, Test performance, Traditional tests, Transm, Treatment interventions, Trichomonas, Trichomonas vaginalis, Trichomonas vaginalis infection, Trichomoniasis, Tropical medicine, Vaginal discharge, Vaginal infections, Vaginal swabs, Vaginal trichomoniasis, Vaginalis, Vaginalis diagnosis, Vaginalis infection, Vaginalis latex agglutination test, latex agglutination, rapid test.
- Teeft :
- Adverse pregnancy, Agglutination, Antenatal clinics, Birth weight, Characteristic morphology, Culture results, Culture testing, Diagnostic comparison, Diagnostic methods, Diagnostic tests, Infection, Inpouch, Inpouch culture, Kappa index, Keppel street, Kwame nkrumah university, Laboratory diagnosis, Latex, Latex agglutination, Latex agglutination test, Latex agglutination testing, London school, London wc1e, Many health settings, Medical sciences, Nucleic acid amplification, Obstet gynecol, Partner notification, Polymerase chain reaction, Predictive value, Prep, Prep culture, Prep microscopy, Prep technique, Present cost, Rapid tests, Remote communities, Reproductive health settings, Risk factors, Specimen collection, Test performance, Traditional tests, Transm, Treatment interventions, Trichomonas, Trichomonas vaginalis, Trichomonas vaginalis infection, Trichomoniasis, Tropical medicine, Vaginal discharge, Vaginal infections, Vaginal swabs, Vaginal trichomoniasis, Vaginalis, Vaginalis diagnosis, Vaginalis infection, Vaginalis latex agglutination test.
Abstract
Objectives: To compare the performance of three diagnostic methods for Trichomonas vaginalis infection—latex agglutination, saline wet mount, and culture. Methods: Vaginal swabs from 3807 women attending antenatal clinics were tested for the presence of T vaginalis by latex agglutination. All positives and the following two negatives were tested by wet preparation and culture. Results: The prevalence of infection by latex agglutination was 5.4%. Using an expanded gold standard based on the wet mount and culture results, the sensitivity of the latex agglutination test was 98.8% (95% CI 95.9 to 99.9) and specificity was 92.1 (89.2 to 94.5). The kappa index for test agreement was 0.93 for latex and culture and 0.88 for latex and wet preparation. Conclusion: The latex agglutination test is a highly sensitive test for detecting T vaginalis infection. It is a simple rapid test and has the potential for use in screening and diagnostic settings.
Url:
DOI: 10.1136/sti.2003.007864
Affiliations:
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adverse pregnancy</term>
<term>Agglutination</term>
<term>Antenatal clinics</term>
<term>Birth weight</term>
<term>Characteristic morphology</term>
<term>Culture results</term>
<term>Culture testing</term>
<term>Diagnostic comparison</term>
<term>Diagnostic methods</term>
<term>Diagnostic tests</term>
<term>Infection</term>
<term>Inpouch</term>
<term>Inpouch culture</term>
<term>Kappa index</term>
<term>Keppel street</term>
<term>Kwame nkrumah university</term>
<term>Laboratory diagnosis</term>
<term>Latex</term>
<term>Latex agglutination</term>
<term>Latex agglutination test</term>
<term>Latex agglutination testing</term>
<term>London school</term>
<term>London wc1e</term>
<term>Many health settings</term>
<term>Medical sciences</term>
<term>Nucleic acid amplification</term>
<term>Obstet gynecol</term>
<term>Partner notification</term>
<term>Polymerase chain reaction</term>
<term>Predictive value</term>
<term>Prep</term>
<term>Prep culture</term>
<term>Prep microscopy</term>
<term>Prep technique</term>
<term>Present cost</term>
<term>Rapid tests</term>
<term>Remote communities</term>
<term>Reproductive health settings</term>
<term>Risk factors</term>
<term>Specimen collection</term>
<term>Test performance</term>
<term>Traditional tests</term>
<term>Transm</term>
<term>Treatment interventions</term>
<term>Trichomonas</term>
<term>Trichomonas vaginalis</term>
<term>Trichomonas vaginalis infection</term>
<term>Trichomoniasis</term>
<term>Tropical medicine</term>
<term>Vaginal discharge</term>
<term>Vaginal infections</term>
<term>Vaginal swabs</term>
<term>Vaginal trichomoniasis</term>
<term>Vaginalis</term>
<term>Vaginalis diagnosis</term>
<term>Vaginalis infection</term>
<term>Vaginalis latex agglutination test</term>
<term>latex agglutination</term>
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<term>Culture results</term>
<term>Culture testing</term>
<term>Diagnostic comparison</term>
<term>Diagnostic methods</term>
<term>Diagnostic tests</term>
<term>Infection</term>
<term>Inpouch</term>
<term>Inpouch culture</term>
<term>Kappa index</term>
<term>Keppel street</term>
<term>Kwame nkrumah university</term>
<term>Laboratory diagnosis</term>
<term>Latex</term>
<term>Latex agglutination</term>
<term>Latex agglutination test</term>
<term>Latex agglutination testing</term>
<term>London school</term>
<term>London wc1e</term>
<term>Many health settings</term>
<term>Medical sciences</term>
<term>Nucleic acid amplification</term>
<term>Obstet gynecol</term>
<term>Partner notification</term>
<term>Polymerase chain reaction</term>
<term>Predictive value</term>
<term>Prep</term>
<term>Prep culture</term>
<term>Prep microscopy</term>
<term>Prep technique</term>
<term>Present cost</term>
<term>Rapid tests</term>
<term>Remote communities</term>
<term>Reproductive health settings</term>
<term>Risk factors</term>
<term>Specimen collection</term>
<term>Test performance</term>
<term>Traditional tests</term>
<term>Transm</term>
<term>Treatment interventions</term>
<term>Trichomonas</term>
<term>Trichomonas vaginalis</term>
<term>Trichomonas vaginalis infection</term>
<term>Trichomoniasis</term>
<term>Tropical medicine</term>
<term>Vaginal discharge</term>
<term>Vaginal infections</term>
<term>Vaginal swabs</term>
<term>Vaginal trichomoniasis</term>
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<term>Vaginalis diagnosis</term>
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<front><div type="abstract" xml:lang="en">Objectives: To compare the performance of three diagnostic methods for Trichomonas vaginalis infection—latex agglutination, saline wet mount, and culture. Methods: Vaginal swabs from 3807 women attending antenatal clinics were tested for the presence of T vaginalis by latex agglutination. All positives and the following two negatives were tested by wet preparation and culture. Results: The prevalence of infection by latex agglutination was 5.4%. Using an expanded gold standard based on the wet mount and culture results, the sensitivity of the latex agglutination test was 98.8% (95% CI 95.9 to 99.9) and specificity was 92.1 (89.2 to 94.5). The kappa index for test agreement was 0.93 for latex and culture and 0.88 for latex and wet preparation. Conclusion: The latex agglutination test is a highly sensitive test for detecting T vaginalis infection. It is a simple rapid test and has the potential for use in screening and diagnostic settings.</div>
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